Background Biomarker-based tests for diagnosing TB currently rely on detecting Mycobacterium tuberculosis (Mtb) antigen-specific cellular responses. While this approach can detect Mtb infection, it is ...

Background: Microarray technology has brought significant advancements to high-throughput analysis, particularly in the comprehensive study of biomolecular interactions involving proteins, peptides, ...

Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Campus Plaine, Université Libre de Bruxelles, CP206/2, B1050 Brussels, Belgium ...

Treatment for peripheral nerve injury remains limited. The inherent differences between motor and sensory nerve fibers in peripheral nerves should be considered to improve the effects of clinical ...

Serial analysis of gene expression (SAGE) uses mRNA from a sample to create complementary DNA (cDNA) fragments for amplification and sequencing using high-throughput sequencing technology. Credit: ...

Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to ...

Background— Apoptosis may contribute to the development of heart failure, but the role of apoptotic signaling initiated by the endoplasmic reticulum in this condition has not been well clarified.

In common to all of the methods described above is the fact that they usually produce binary data: proteins are reported either to 'interact' or 'not interact'. As we seek to extract biological ...

Protein-binding microarray (PBM) technology provides a rapid, high-throughput means of characterizing the in vitro DNA-binding specificities of transcription factors (TFs). Using high-density, ...

The melanocortin-1 receptor (MC1R) is a key regulator of pigmentation in mammals and is tightly linked to an increased risk of skin cancers, including melanoma, in humans. Physiologically activated by ...

Students model how scientists use DNA microarrays to determine levels of gene expression in breast cancer patients, and then choose treatments based on what they learn. Normal-functioning DNA codes ...

Here, we describe a high-throughput approach involving a single-round PCR, followed by in vivo recombination cloning and in vitro expression, that can rapidly lead to the generation of complete ...