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FIJI ImageJ was employed to convert the Z-stacks (50 μm total images, 1 μm each interval) into single projection images using average intensity projection. Image analysis For MN formation analysis, ...

Do this in two stages: install uv to easily manage packages (you may already have it installed), then install stackEDS-TW itself. A folder picker opens - choose a directory containing your .tif ...

Correspondence to Dr Venu G Pillarisetty, Surgery, University of Washington, Seattle, WA 98195, USA; vgp{at}uw.edu; Dr Qiang Tian, 197 Ruijin Er Road, Shanghai, People's Republic of China 200025; ...