Dissolved oxygen was monitored online with normal and trace micro-optodes (types PSt1 and TOS7, Presens) with detection limits of 125 and 20 nM, respectively, and a response time of 7 s. In October ...

The mounting medium was cured for at least 48 h in Prolong-Gold before imaging. STED images were acquired using a 100×, 1.4 NA oil objective on a Leica SP8 X STED microscope. A white-light laser ...