Analyses in the remainder of the section were conducted using R (v3.6) 46 with Seurat (v3.1.5) 47, 48. Bioinformatics plots were generated using ggplot2 (v3.3.1) 49, and heat maps with pheatmap ...

The guide RNA sequence targeting exon 1 of mouse Sirt7 homology sequence was designed and cloned into pSpCas9 (BB)−2A-GFP (Addgene, 48138). After Sanger sequencing verification, 0.1 million cells ...

While global output has advanced consistently and moderately, Bahrain-affiliated publications shifted from prolonged low productivity to a rapid increase after 2010, with most documents appearing in ...