NanoDrop One/OneC超微量紫外分光光度计可以应用于qPCR/RT-qPCR的核酸质控中,其中的Acclaro™智能样本检测技术能够检测存在于DNA ...
NanoDrop One/OneC超微量紫外分光光度计可以应用于qPCR/RT-qPCR的核酸质控中,其中的Acclaro™智能样本检测技术能够检测存在于DNA ...
荧光定量PCR(qPCR)是目前病原检测领域使用最为广泛的核酸检测方法。尤其是非洲猪瘟和新冠发生后,qPCR检测得到了极大的普及,对于刚刚进入qPCR检测领域的人,很容易钻入了“唯Ct值”的牛角尖,今天我们就聊聊qPCR中的Ct值。 一、Ct值的基本概念 1、什么是Ct ...
为什么更换仪器后会出现BadRox报错? BadRox报错是因为更换qPCR仪器后,试剂中添加的ROX浓度与仪器不匹配导致的。 什么是ROX? ROX是一种惰性参比荧光染料,能被qPCR仪检测到,但其荧光信号值在qPCR反应中基本保持不变,不会随着qPCR扩增而增强,不会对qPCR反应 ...
精准识别表皮生长因子受体(EGFR)基因突变对于非小细胞肺癌(NSCLC)患者的个体化治疗至关重要。当下市场上,存在多种用于检测这些基因突变的方法和仪器,然而其检测性能参差不齐,尤其是在检测少见 EGFR 基因突变方面,差异更为明显。近日,Lung Cancer ...
Real-time quantitative PCR (RT-qPCR or qPCR) is revolutionizing many aspects of molecular biology and becoming the gold standard for accurate, sensitive, and fast quantification of gene expression.
The scope of real-time quantitative (qPCR) assays for gene-expression analysis in research applications and for sequence-specific nucleic acid detection for diagnostic purposes continues to grow.
When working in a lab, launching a quantitative PCR (qPCR) experiment demands a comprehensive understanding of complicated protocols, principles, and methodologies. Analyzing the large amounts of ...
Leading PCR and qPCR technology now available for high-throughput applications: With the Biometra TRobot II and qTOWER³ auto, Analytik Jena presents two new thermal cyclers for fully automated PCR and ...
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