生物通

定量PCR中SYBR Green I的替代[选购宝典]

定量PCR中的染料通常是SYBR Green I，但它也并非完美。SYBR Green I对PCR有抑制作用，在实验中的使用浓度很低，且与富含GC的序列优先结合。于是，研究人员也在不断尝试新品，来替代SYBR Green I。 定量PCR一般有两条路，一条是探针，另一条是染料。因染料是普遍适用 ...
生物通

定量PCR技术报告之三：如何选择荧光定量检测技术(I)之SYBR Green I系列 ...

对于刚刚初接触定量PCR技术的研究人员来说，仪器是老板选的，没得挑；试剂就不同了，荧光检测手段五花八门，新产品不断推陈出新，使得你的选择玲琅满目——如何在实验室已有的条件下，根据自己的实验需要，挑选合适的荧光检测试剂也就成为了决定定量 ...
technologynetworks

Roche Diagnostics Licenses Rights to Use SYBR Green in Real Time PCR to Exiqon

Roche Diagnostics and Exiqon A/S announced that they have entered into a license agreement for use of SYBR Green reagent in PCR. Under the terms of the agreement, Exiqon obtains from Roche a ...
EurekAlert!

Development of a SYBR green-based RT-qPCR assay for the detection and quantification of ...

Announcing a new article publication for Zoonoses journal. Lone Star virus (LSV) is a newly characterized arbovirus with pathogenic potential. However, no detection methods are available to ...