The effectiveness of CAR T cell therapy against a variety of cancers, including solid tumors, could be boosted greatly by using CRISPR-Cas9 technology to knock out the gene for CD5, a protein found on ...

Using CRISPR/Cas9, the team engineered lung cancer cells with the R34G mutation and successfully knocked out NRF2. This restored sensitivity to chemotherapy drugs such as carboplatin and paclitaxel.

Cas9蛋白是一种以RNA为导向的DNA内切酶，是一种操纵基因组的强大工具[1]。更多的研究者获益于基于CRISPR的遗传筛查，对已确定的基因有更多基因功能表型的认识[2]。早在2013年，海外的两个研究团队分别在Science杂志同一期上发表了CRISPR文库的相关研究[3,4]。这是首次关于CRISPR文库的研究，显示出比shRNA文库更有效的筛选效果。但在文库设计初始，对sgRNA活性规则知 ...

Twelve people whose stomach or colon cancers had defeated every available treatment were infused with their own immune cells, ...

“While recent single-cell multiomics studies and CRISPR screens in murine NK cells have expanded our understanding, actionable genomic targets in primary human NK cells have remained largely ...

EditCo Bio and Promega have announced a strategic licensing agreement that grants EditCo access to Promega’s HiBiT, HaloTag, and NanoLuc technologies. Under the terms of the arrangement, EditCo will ...

Northwestern Medicine investigators have developed a new avenue to reprogram compromised immune cells to act against tumors, according to a study published in Science Advances. Shana Kelley, Ph.D., ...

Thanks to CRISPR, our medical specialists will soon have unprecedented control over how they treat and prevent some of our most challenging genetic disorders and diseases. CRISPR (Clustered Regularly ...